Chapter 1 history and biology of erythropoietin in. Epo production was carefully carried out under the guidelines of good industrial largescale practice gilsp, for drug production using recombinant dna technology. In the case of epo production, the sequence of amino acids, as well as the amount of glycosylation, must be correct to achieve the desired efficacy in vivo. The production of recombinant human erythropoietin. The hormone erythropoietin epo is essential for red blood cell rbc production. Two radiolabeled pools of oligonucleotide probes were designed, based on amino acid sequence information obtained from human urinary epo. The introduction of recombinant human erythropoietin rhuepo has revolutionised the treatment of patients with anaemia of chronic renal disease. Erythropoietin is a glycoprotein with a molecular weight of. This chapter describes one method of epo production using cells genetically engineered to secrete recombinant human erythropoietin rhuepo.
The hormone epo is an essential growth factor for the production of red blood cells. The mech anisms underlying the liver to kidney switch of ep. Erythropoietin, recombinant, human drug information. In vitro production of erythropoietin by kidneys perfused with a serumfree solution. Similarly, the observation that erythropoietin gene expression in several other organs is modulated by anemia andor hypoxia suggest that the hif. Erythropoietin in the pathogenesis and treatment of the anemia. Anemia induces accumulation of erythropoietin mrna in the kidney and liver. Recombinant erythropoietin therapy in renal and nonrenal. Sites of erythropoietin production kidney international. Clinical studies have demonstrated that rhuepo is also useful in various nonuraemic conditions including haematological and oncological disorders. Endogenous erythropoietin production may be suppressed by chronic administration of recombinant epoetin 22.
Commercial production of recombinant erythropoietins. Regulation of erythropoiesis by hypoxiainducible factors. Get a printable copy pdf file of the complete article 429k, or click on a page image below to browse page by page. Costeffective indirect elisa method for determination of recombinant human erythropoietin in production streams. Large scale production of recombinant human erythropoietin. In the first step of epo production in culture medium, frozen master clean room ground plan 9. Includes erythropoietin, recombinant, human side effects, interactions and indications. Erythropoietin epo, a heavily glycosylated protein, is a major stimulatory factor in erythropoiesis. Molecular biology of erythropoietin kidney international.
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